Molecular Characterization of an IncFII_k Plasmid Co-harboring bla _IMP-26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate.

ABSTRACT: Carbapenems and tigecycline are two important classes of antimicrobial agents to treat the infections caused by Enterobacterales. Here, we reported a plasmid carrying both bla _IMP-26 and tet(A) variant in clinical Klebsiella pneumoniae KP-1572. MIC results showed that K. pneumonia KP-1572 was resistant to a wide range of antimicrobials. The bla _IMP-26 and tet(A) variant were located on an identical plasmid, which was indicated by S1-PFGE and southern blotting hybridization and can be successfully transferred by electroporation. Whole-plasmid sequencing and analysis revealed that a 142,993-bp-sized plasmid, designated pIMP1572, contains an IncFII_k backbone and a variable region harboring bla _IMP-26

SUBMITTER: Yao H 

PROVIDER: S-EPMC7393768 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature