Osterix-mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model.

ABSTRACT: The process of new bone formation following trauma requires the temporal recruitment of cells to the site, including mesenchymal stem cells, preosteoblasts, and osteoblasts, the latter of which deposit minerals. Hence, bone repair, a process that is assessed by the extent of mineralization within the defect, can take months before it is possible to determine if a treatment is successful. Here, a fluorescently tagged Osterix, an early key gene in the bone formation cascade, is used as a predictive measure of bone formation. Using a calvarial defect model in mice, the ability to noninvasively track the Osterix transcription factor in an Osterix-mCherry mouse model is evaluated as a measure for bone formation following treatment with recombinant human Bone-Morphogenetic-Protein 2 (rhBMP-2). T

SUBMITTER: Strecker SE 

PROVIDER: S-EPMC7393777 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature