Direct lineage tracing reveals Activin-a potential for improved pancreatic homing of bone marrow mesenchymal stem cells and efficient ß-cell regeneration in vivo.

ABSTRACT:

Background

Despite the potential, bone marrow-derived mesenchymal stem cells (BMSCs) show limitations for beta (ß)-cell replacement therapy due to inefficient methods to deliver BMSCs into pancreatic lineage. In this study, we report TGF-ß family member protein, Activin-a potential to stimulate efficient pancreatic migration, enhanced homing and accelerated ß-cell differentiation.

Methods

Lineage tracing of permanent green fluorescent protein (GFP)- tagged donor murine BMSCs transplanted either alone or in combination with Activin-a in diabetic mice displayed potential ß-cell regeneration and reversed diabetes.

Results

Pancreatic histology of Activin-a treated recipient mice reflected high GFP⁺BMSC infiltration into damaged pancreas with normalized fasting

SUBMITTER: Dadheech N 

PROVIDER: S-EPMC7393856 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature