Selective Translation of Low Abundance and Upregulated Transcripts in Halobacterium salinarum.

ABSTRACT: When organisms encounter an unfavorable environment, they transition to a physiologically distinct, quiescent state wherein abundant transcripts from the previous active growth state continue to persist, albeit their active transcription is downregulated. In order to generate proteins for the new quiescent physiological state, we hypothesized that the translation machinery must selectively translate upregulated transcripts in an intracellular milieu crowded with considerably higher abundance transcripts from the previous active growth state. Here, we have analyzed genome-wide changes in the transcriptome (RNA sequencing [RNA-seq]), changes in translational regulation and efficiency by ribosome profiling across all transcripts (ribosome profiling [Ribo-seq]), and protein level changes in as

SUBMITTER: Lopez Garcia de Lomana A 

PROVIDER: S-EPMC7394353 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature