Microbiome and Metagenome Analyses of a Closed Habitat during Human Occupation.

ABSTRACT: Microbial contamination during long-term confinements of space exploration presents potential risks for both crew members and spacecraft life support systems. A novel swab kit was used to sample various surfaces from a submerged, closed, analog habitat to characterize the microbial populations. Samples were collected from various locations across the habitat which were constructed from various surface materials (linoleum, dry wall, particle board, glass, and metal), and microbial populations were examined by culture, quantitative PCR (qPCR), microbiome 16S rRNA gene sequencing, and shotgun metagenomics. Propidium monoazide (PMA)-treated samples identified the viable/intact microbial population of the habitat. The cultivable microbial population ranged from below the detection limit to 10

SUBMITTER: Malli Mohan GB 

PROVIDER: S-EPMC7394354 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature