Molecular characterization of B. anthracis isolates from the anthrax outbreak among cattle in Karnataka, India.

ABSTRACT:

Background

Anthrax, a zoonotic disease is caused by the Gram positive bacterium Bacillus anthracis. During January 2013, an anthrax outbreak among cattle was reported in Gundlupet Taluk, neighboring Bandipur National Park and tiger reserve, India. The present study aims at the molecular identification and characterization of 12 B. anthracis isolates from this outbreak by 16S rRNA gene sequencing, screening B. anthracis specific prophages and chromosomal markers, protective antigen (pag) gene and canonical single nucleotide polymorphism (canSNP) analysis to subtype the isolates into one of the twelve globally identified clonal sub-lineages of B. anthracis.

Results

These isolates had identical 16S rDNA nucleotide sequences with B. anthracis specific dual peaks showing mixed ba

SUBMITTER: Roonie A 

PROVIDER: S-EPMC7394690 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature