Composition of Caenorhabditis elegans extracellular vesicles suggests roles in metabolism, immunity, and aging.

ABSTRACT: The nematode Caenorhabditis elegans has been instrumental in the identification of evolutionarily conserved mechanisms of aging. C. elegans also has recently been found to have evolutionarily conserved extracellular vesicle (EV) signaling pathways. We have been developing tools that allow for the detailed study of EV biology in C. elegans. Here we apply our recently published method for high specificity purification of EVs from C. elegans to carry out target-independent proteomic and RNA analysis of nematode EVs. We identify diverse coding and non-coding RNA and protein cargo types commonly found in human EVs. The EV cargo spectrum is distinct from whole worms, suggesting that protein and RNA cargos are actively recruited to EVs. Gene ontology analysis revealed C. elegans EVs are enriched

SUBMITTER: Russell JC 

PROVIDER: S-EPMC7394990 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature