Overexpression of phosphatidylserine synthase IbPSS1 affords cellular Na⁺ homeostasis and salt tolerance by activating plasma membrane Na⁺/H⁺ antiport activity in sweet potato roots.

ABSTRACT: Phosphatidylserine synthase (PSS)-mediated phosphatidylserine (PS) synthesis is crucial for plant development. However, little is known about the contribution of PSS to Na⁺ homeostasis regulation and salt tolerance in plants. Here, we cloned the IbPSS1 gene, which encodes an ortholog of Arabidopsis AtPSS1, from sweet potato (Ipomoea batatas (L.) Lam.). The transient expression of IbPSS1 in Nicotiana benthamiana leaves increased PS abundance. We then established an efficient Agrobacterium rhizogenes-mediated in vivo root transgenic system for sweet potato. Overexpression of IbPSS1 through this system markedly decreased cellular Na⁺ accumulation in salinized transgenic roots (TRs) compared with adventitious roots. The ove

SUBMITTER: Yu Y 

PROVIDER: S-EPMC7395154 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature