Complete microviscosity maps of living plant cells and tissues with a toolbox of targeting mechanoprobes.

ABSTRACT: Mechanical patterns control a variety of biological processes in plants. The microviscosity of cellular structures effects the diffusion rate of molecules and organelles, thereby affecting processes such as metabolism and signaling. Spatial variations in local viscosity are also generated during fundamental events in the cell life cycle. While crucial to a complete understanding of plant mechanobiology, resolving subcellular microviscosity patterns in plants has remained an unsolved challenge. We present an imaging microviscosimetry toolbox of molecular rotors that yield complete microviscosity maps of cells and tissues, specifically targeting the cytosol, vacuole, plasma membrane, and wall of plant cells. These boron-dipyrromethene (BODIPY)-based molecular rotors are rigidochromic by mean

SUBMITTER: Michels L 

PROVIDER: S-EPMC7395454 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature