Peptidylglycine α-amidating monooxygenase is required for atrial secretory granule formation.

ABSTRACT: The discovery of atrial secretory granules and the natriuretic peptides stored in them identified the atrium as an endocrine organ. Although neither atrial nor brain natriuretic peptide (ANP, BNP) is amidated, the major membrane protein in atrial granules is peptidylglycine α-amidating monooxygenase (PAM), an enzyme essential for amidated peptide biosynthesis. Mice lacking cardiomyocyte PAM (Pam^Myh6-cKO/cKO) are viable, but a gene dosage-dependent drop in atrial ANP and BNP content occurred. Ultrastructural analysis of adult Pam^Myh6-cKO/cKO atria revealed a 13-fold drop in the number of secretory granules. When primary cultures of Pam^{0-Cre-cKO/cKO} atrial myocytes (no Cre recombinase, PAM floxed) were transduced with Cre-GFP lenti

SUBMITTER: Back N 

PROVIDER: S-EPMC7395455 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature