Spermatogenesis is normal in Tex33 knockout mice.

ABSTRACT: Testis expressed gene 33 (Tex33) is a recently reported testis-specific gene and it is evolutionarily conserved in vertebrates. The Tex33 expression is found in cytoplasm of round spermatids in Mus musculus. However, the in vivo function of Tex33 remains unknown. In this study, we made a 62bp in frame deletion on Exon2 of Tex33 gene by CRISPR/Cas9 in C57B/L6 mouse, which cause frame shift mutation of Tex33 gene. Tex33 ^-/-adult male were fertile, and there is no significant change on litter size compared with male wildtype (Tex33 ^+/+) adult. Besides, no overt differences were found in testis/body weight ratios, testicular/epididymal tissue morphology, sperm counts, sperm morphology and spermatozoa motility in adult Te

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC7395601 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature