Identification and Validation of Reference Genes Selection in Ovarian Cancer Exposed to Hypoxia.

ABSTRACT:

Introduction

Hypoxia-mediated tumor metastasis, progression and drug resistance are major clinical challenges in ovarian cancer. Meanwhile, the genetic basis of these traits is still not clear. RT-qPCR, as an efficient and sensitive gene expression technique, has been widely used for gene analyses, providing a basis for in-depth understanding of molecular changes in different microenvironments. However, there is currently a lack of suitable reference genes to normalize the data associated with hypoxia in ovarian cancer cells.

Methods

A systematic method is needed to select the most suitable reference gene. Here, eight candidate reference genes (GAPDH, β-actin, 18S RNA, TUBB, PPIA, TBP, RPL13A and SDHA) from humans were selected to assess their expression levels in SKOV3 cell

SUBMITTER: Yan W 

PROVIDER: S-EPMC7395691 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature