Rapid counting and spectral sorting of live coral larvae using large-particle flow cytometry.

ABSTRACT: Research with coral embryos and larvae often requires laborious manual counting and sorting of individual specimens, usually via microscopy. Because many coral species spawn only once per year during a narrow temporal window, sample processing is a time-limiting step for research on the early life-history stages of corals. Flow cytometry, an automated technique for measuring and sorting particles, cells, and cell-clusters, is a potential solution to this bottleneck. Yet most flow cytometers do not accommodate live organisms of the size of most coral embryos (> 250 µm), and sample processing is often destructive. Here we tested the ability of a large-particle flow cytometer with a gentle pneumatic sorting mechanism to process and spectrally sort live and preserved Montipora capitata coral e

SUBMITTER: Randall CJ 

PROVIDER: S-EPMC7395729 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature