Evaluation of a novel multiplexed assay for determining IgG levels and functional activity to SARS-CoV-2.

ABSTRACT:

Background

The emergence of SARS-CoV-2 has led to the development of serological assays that could aid in an understanding of the burden of COVID-19 disease. Many available tests lack rigorous evaluation and therefore results may be misleading.

Objectives

The aim of this study was to assess the performance of a novel multiplexed immunoassay for the simultaneous detection of antibodies against SARS-CoV-2 trimeric spike (S), spike receptor binding domain (RBD), spike N terminal domain and nucleocapsid antigen and a novel pseudo-neutralisation assay.

Methods

A multiplexed solid-phase chemiluminescence assay (Meso Scale Discovery) was evaluated for the simultaneous detection of IgG binding to four SARS-CoV-2 antigens and the quantification of antibody-induced ACE-2 bindin

SUBMITTER: Johnson M 

PROVIDER: S-EPMC7396134 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature