DNA from mollusc shell: a valuable and underutilised substrate for genetic analyses.

ABSTRACT: Mollusc shells are an abundant resource that have been long used to predict the structures of ancient ecological communities, examine evolutionary processes, reconstruct paleoenvironmental conditions, track and predict responses to climatic change, and explore the movement of hominids across the globe. Despite the ubiquity of mollusc shell in many environments, it remains relatively unexplored as a substrate for molecular genetic analysis. Here we undertook a series of experiments using the New Zealand endemic greenshell mussel, Perna canaliculus, to explore the utility of fresh, aged, beach-cast and cooked mollusc shell for molecular genetic analyses. We find that reasonable quantities of DNA (0.002-21.48 ng/mg shell) can be derived from aged, beach-cast and cooked mussel shell and

SUBMITTER: Ferreira S 

PROVIDER: S-EPMC7396136 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature