In Vitro Culture of Human Corneal Endothelium on Non-Mulberry Silk Fibroin Films for Tissue Regeneration.

ABSTRACT:

Purpose

The purpose of this study was to determine if non-mulberry varieties of silk are suitable for the culture of corneal endothelium (CE).

Methods

Aqueous silk fibroin derived from Philosamia ricini (PR), Antheraea assamensis (AA), and Bombyx mori (BM) were cast as approximately 15 µm films with and without pores on which human CE cells were cultured. Tensile strength, elasticity, transmittance in visible range, and degradation properties of the films were characterised. Adhesion of CE to the silk films was quantified using MTT assay in addition to quantifying the number and area of focal adhesions using paxillin. Expression of CE markers was determined at the gene and protein levels using PCR and immunostaining, respectively. Barrier integrity of the cultured cells was

SUBMITTER: Ramachandran C 

PROVIDER: S-EPMC7396167 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature