In situ Vaccine Plus Checkpoint Blockade Induces Memory Humoral Response.

ABSTRACT: In a syngeneic murine melanoma (MEL) model, we recently reported an in situ vaccination response to combined radiation (RT) and intra-tumoral (IT) injection of anti-GD2 hu14. 18-IL2 immunocytokine (IC). This combined treatment resulted in 71% complete and durable regression of 5-week tumors, a tumor-specific memory T cell response, and augmented response to systemic anti-CTLA-4 antibody checkpoint blockade. While the ability of radiation to diversify anti-tumor T cell response has been reported, we hypothesize that mice rendered disease-free (DF) by a RT-based ISV might also exhibit a heightened B cell response. C57BL/6 mice were engrafted with 2 × 10⁶ GD2+ B78 MEL and treated at a target tumor size of ~200 mm³ with 12 Gy RT, IT-IC on day (D)6-D10, and anti-CTL

SUBMITTER: Baniel CC 

PROVIDER: S-EPMC7396490 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature