High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming.

ABSTRACT: Pectate lyases play an essential role in textiles, animal feed, and oil extraction industries. Pichia pastoris can be an ideal platform for pectate lyases production, and BspPel (a thermo-alkaline pectate lyase from Bacillus sp. RN1) was overexpressed by combined strategies, reaching 1859 U/mL in a 50 L fermentator. It displayed the highest activity at 80°C, and maintained more than 60% of the activity between 30 and 70°C for 1 h. It showed an optimal pH of 10.0, and exhibited remarkable stability over a wider pH range (3.0-11.0), retaining more than 80.0% of enzyme activity for 4 h. The K _m and k _cat of BspPel on PGA (polygalacturonic acid) was 2.19 g L^-1 and 116.1 s^-1, respectively. The activity was sig

SUBMITTER: Zheng X 

PROVIDER: S-EPMC7396651 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature