Genome Editing of eIF4E1 in Tomato Confers Resistance to Pepper Mottle Virus.

ABSTRACT: Many of the recessive virus-resistance genes in plants encode eukaryotic translation initiation factors (eIFs), including eIF4E, eIF4G, and related proteins. Notably, eIF4E and its isoform eIF(iso)4E are pivotal for viral infection and act as recessive resistance genes against various potyviruses in a wide range of plants. In this study, we used Clustered Regularly Interspaced Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9)-mediated targeted mutagenesis to test whether novel sequence-specific mutations at eIF4E1 in Solanum lycopersicum (tomato) cv. Micro-Tom could confer enhanced resistance to potyviruses. This approach produced heritable homozygous mutations in the transgene-free E₁ generation. Sequence analysis of eIF4E1 from E<

SUBMITTER: Yoon YJ 

PROVIDER: S-EPMC7396686 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature