Golgi-localized exo-β1,3-galactosidases involved in cell expansion and root growth in Arabidopsis.

ABSTRACT: Plant arabinogalactan proteins (AGPs) are a diverse group of cell surface- and wall-associated glycoproteins. Functionally important AGP glycans are synthesized in the Golgi apparatus, but the relationships among their glycosylation levels, processing, and functionalities are poorly understood. Here, we report the identification and functional characterization of two Golgi-localized exo-β-1,3-galactosidases from the glycosyl hydrolase 43 (GH43) family in Arabidopsis thaliana GH43 loss-of-function mutants exhibited root cell expansion defects in sugar-containing growth media. This root phenotype was associated with an increase in the extent of AGP cell wall association, as demonstrated by Yariv phenylglycoside dye quantification and comprehensive microarray polymer profiling of seque

SUBMITTER: Nibbering P 

PROVIDER: S-EPMC7397118 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature