Extraction of Lipids from Liquid Biological Samples for High-Throughput Lipidomics.

ABSTRACT: Extraction of the lipid fraction is a key part of acquiring lipidomics data. High-throughput lipidomics, the extraction of samples in 96w plates that are then run on 96 or 384w plates, has particular requirements that mean special development work is needed to fully optimise an extraction method. Several methods have been published as suitable for it. Here, we test those methods using four liquid matrices: milk, human serum, homogenised mouse liver and homogenised mouse heart. In order to determine the difference in performance of the methods as objectively as possible, we used the number of lipid variables identified, the total signal strength and the coefficient of variance to quantify the performance of the methods. This showed that extraction methods with an aqueous component were gene

SUBMITTER: Furse S 

PROVIDER: S-EPMC7397209 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature