Pro12Ala PPAR-?2 and +294T/C PPAR-? Polymorphisms and Association with Metabolic Traits in Teenagers from Northern Mexico.

ABSTRACT: Peroxisome proliferator-activated receptors (PPARs) play roles in glucose and lipid metabolism regulation. Pro12Ala PPAR-?2 and +294T/C PPAR-? have been associated with dyslipidemia, hyperglycemia and high body mass index (BMI). We compared metabolic traits and determined associations with Pro12Ala PPAR-?2 or +294T/C PPAR-? polymorphism among teenagers from different ethnicity. Four hundred and twelve samples with previous biochemical and biometric measurements were used. Genomic DNA from peripheral blood was extracted and analyzed by end-point PCR for Pro12Ala PPAR-?2. The +294T/C PPAR-? PCR product was also digested with Bsl I. Two genotype groups were formed: major allele homozygous and minor allele carriers. Pro12Ala PPAR-?2 G minor allele frequencies were: 10% in Mestizo-1, 19% in Mestizo-2, 23% in Tarahumara, 12% in Mennonite, and 17% in the total studied population. The +294T/C PPAR-? C minor allele frequencies were: 18% in Mestizo-1, 20% in Mestizo-2, 6% in Tarahumara, 13% in Mennonite, and 12% in the total studied population. Teenagers with PPAR-?2 G allele showed a greater risk for either high waist/height ratio or low high-density lipoprotein; and, also had lower total cholesterol. Whereas, PPAR-?2 G allele showed lower overweight/obesity phenotype (BMI Z-score) frequency, PPAR-? C allele was a risk factor for it. Metabolic traits were associated with both PPAR polymorphisms.

SUBMITTER: Carrillo-Venzor MA 

PROVIDER: S-EPMC7397260 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature