Proteomic comparison of osteoarthritic and reference human menisci using data-independent acquisition mass spectrometry.

ABSTRACT:

Objective

Recent research in knee osteoarthritis (OA) highlights the role of the meniscus in OA pathology. Our aim was to compare the proteomes of medial and lateral menisci from end-stage medial compartment knee OA patients, with reference menisci from knee-healthy deceased donors, using mass spectrometry.

Design

Tissue plugs of Ø3 mm were obtained from the posterior horns of the lateral and medial menisci from one knee of 10 knee-healthy deceased donors and 10 patients undergoing knee replacement. Proteins were extracted and prepared for mass spectrometric analysis. Statistical analysis was conducted on abundance data that was log₂-transformed, using a linear mixed effects model and evaluated using pathway analysis.

Results

We identified a total of 835 pr

SUBMITTER: Folkesson E 

PROVIDER: S-EPMC7397514 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature