MCL1 Is Required for Maintenance of Intestinal Homeostasis and Prevention of Carcinogenesis in Mice.

ABSTRACT:

Background & aims

Intestinal epithelial homeostasis depends on a tightly regulated balance between intestinal epithelial cell (IEC) death and proliferation. While the disruption of several IEC death regulating factors result in intestinal inflammation, the loss of the anti-apoptotic BCL2 family members BCL2 and BCL2L1 has no effect on intestinal homeostasis in mice. We investigated the functions of the antiapoptotic protein MCL1, another member of the BCL2 family, in intestinal homeostasis in mice.

Methods

We generated mice with IEC-specific disruption of Mcl1 (Mcl1^ΔIEC mice) or tamoxifen-inducible IEC-specific disruption of Mcl1 (i-Mcl1^ΔIEC mice); these mice and mice with full-length Mcl1 (controls) were raised under normal or germ-free conditions. Mic

SUBMITTER: Healy ME 

PROVIDER: S-EPMC7397524 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature