Genipin suppression of growth and metastasis in hepatocellular carcinoma through blocking activation of STAT-3.

ABSTRACT:

Background

The signal transducer and activator of transcription-3 (STAT-3) can facilitate cancer progression and metastasis by being constitutively active via various signaling. Abundant evidence has indicated that STAT-3 may be a promising molecular target for cancer treatment.

Methods

In this study, a dual-luciferase assay-based screening of 537 compounds for STAT-3 inhibitors of hepatocellular carcinoma (HCC) cells was conducted, leading to the identification of genipin. Effects of genipin on HCC were assessed in a patient-derived xenograft nude mice model. Western blotting assay, chromatin immunoprecipitation (ChIP) assay, molecular docking study, tube formation assay, three-dimensional top culture assay, histological examination, and immunofluorescence were utilized to

SUBMITTER: Hong M 

PROVIDER: S-EPMC7397684 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature