Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation.

ABSTRACT: We examined the effect of ice-binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10^-1  ~ 10⁴  μg/ml) and AFPIII (10^-1  ~ 10⁴  μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence micr

SUBMITTER: Jang H 

PROVIDER: S-EPMC7397894 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature