Multiplex Imaging of Polymicrobial Communities-Murine Models to Study Oral Microbiome Interactions.

ABSTRACT: Similar to other mucosal surfaces of the body, the oral cavity hosts a diverse microbial flora that live in polymicrobial biofilm communities. It is the ecology of these communities that are the primary determinants of oral health (symbiosis) or disease (dysbiosis). As such, both symbiosis and dysbiosis are inherently polymicrobial phenomena. In an effort to facilitate studies of polymicrobial communities within rodent models, we developed a suite of synthetic luciferases suitable for multiplexed in situ analyses of microbial ecology and specific gene expression. Using this approach, it is feasible to noninvasively measure multiple luciferase signals in vivo with both spatial and temporal resolution. In the following chapter, we describe the relevant details and protocols used to establish

SUBMITTER: Kreth J 

PROVIDER: S-EPMC7398006 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature