Isolation and whole-genome sequencing of Pseudomonas sp. RIT 623, a slow-growing bacterium endowed with antibiotic properties.

ABSTRACT:

Objective

There is an urgent need for the discovery and/or development of novel antibiotics. We report an exploration of "slow"-growing bacteria, which can be difficult to isolate using rich media as they are usually outcompeted by "fast"-growing bacteria, as potential sources of novel antimicrobials.

Results

Pseudomonas sp. RIT 623 was isolated using pond water agar from a pond located on the campus of the Rochester Institute of Technology (RIT). The genome was sequenced and analyzed for potential secondary metabolite gene clusters. Bioinformatics analysis revealed 14 putative gene clusters predicted to encode pathways for the anabolism of secondary metabolites. Ethyl acetate extracts from spent growth medium of Pseudomonas sp. RIT 623 were tested against two Gram-negative

SUBMITTER: Steiner KK 

PROVIDER: S-EPMC7398229 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature