Complement inhibitor Crry expression in mouse placenta is essential for maintaining normal blood pressure and fetal growth.

ABSTRACT: Many circumstantial evidences from human and animal studies suggest that complement cascade dysregulation may play an important role in pregnancy associated complications including preeclampsia. Deletion of rodent specific complement inhibitor gene, Complement Receptor 1-related Gene/Protein y (Crry) produces embryonic lethal phenotype due to complement activation. It is not clear if decreased expression of Crry during pregnancy produces hypertensive phenotype. We downregulated Crry in placenta by injecting inducible lentivialshRNA vectors into uterine horn of pregnant C57BL/6 mice at the time of blastocyst hatching. Placenta specific downregulation of Crry without significant loss of embryos was achieved upon induction of shRNA using an optimal doxycycline dose at mid gestation. Crry down

SUBMITTER: Banadakoppa M 

PROVIDER: S-EPMC7398533 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature