Impact of time and temperature on gut microbiota and SCFA composition in stool samples.

ABSTRACT: Gut dysbiosis has been implicated in the pathophysiology of a growing number of non-communicable diseases. High through-put sequencing technologies and short chain fatty acid (SCFA) profiling enables surveying of the composition and function of the gut microbiota and provide key insights into host-microbiome interactions. However, a methodological problem with analyzing stool samples is that samples are treated and stored differently prior to submission for analysis potentially influencing the composition of the microbiota and its metabolites. In the present study, we simulated the sample acquisition of a large-scale study, in which stool samples were stored for up to two days in the fridge or at room temperature before being handed over to the hospital. To assess the influence of time and

SUBMITTER: Cunningham JL 

PROVIDER: S-EPMC7398539 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature