Ontology highlight
ABSTRACT:
SUBMITTER: Zhang N
PROVIDER: S-EPMC7398574 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Journal of visualized experiments : JoVE 20200710 161
Mass spectrometry (MS)-based sequencing approaches have been shown to be useful in direct sequencing RNA without the need for a complementary DNA (cDNA) intermediate. However, such approaches are rarely applied as a de novo RNA sequencing method, but used mainly as a tool that can assist in quality assurance for confirming known sequences of purified single-stranded RNA samples. Recently, we developed a direct RNA sequencing method by integrating a 2-dimensional mass-retention time hydrophobic e ...[more]