Optimal identification of human conventional and nonconventional (CRTH2^-IL7Rα^-) ILC2s using additional surface markers.

ABSTRACT:

Background

Human type 2 innate lymphoid cells (ILC2s) are identified by coupled detection of CRTH2 and IL7Rα on lineage negative (Lin^-) cells. Type 2 cytokine production by CRTH2^-IL7Rα^- innate lymphoid cells (ILCs) is unknown.

Objective

We sought to identify CRTH2^-IL7Rα^- type 2 cytokine-producing ILCs and their disease relevance.

Methods

We studied human blood and lung ILCs from asthmatic and control subjects by flow cytometry, ELISA, RNA sequencing, quantitative PCR, adoptive transfer to mice, and measurement of airway hyperreactivity by Flexivent.

Results

We found that IL-5 and IL-13 were expressed not only by CRTH2⁺ but also by CRTH2^-IL7Rα⁺ and CRTH2^-IL7Rα

SUBMITTER: Liu S 

PROVIDER: S-EPMC7398840 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature