Precision Engineering of the Transcription Factor Cre1 in Hypocrea jecorina (Trichoderma reesei) for Efficient Cellulase Production in the Presence of Glucose.

ABSTRACT: In Trichoderma reesei, carbon catabolite repression (CCR) significantly downregulates the transcription of cellulolytic enzymes, which is usually mediated by the zinc finger protein Cre1. It was found that there is a conserved region at the C-terminus of Cre1/CreA in several cellulase-producing fungi that contains up to three continuous S/T phosphorylation sites. Here, S387, S388, T389, and T390 at the C-terminus of Cre1 in T. reesei were mutated to valine for mimicking an unphosphorylated state, thereby generating the transformants Tr_Cre1^S387V, Tr_Cre1^S388V, Tr_Cre1^T389V, and Tr_Cre1^T390V, respectively. Transcription of cel7a in Tr_ Cre1^S388V was markedly higher than that of the

SUBMITTER: Han L 

PROVIDER: S-EPMC7399057 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature