Detecting protein and post-translational modifications in single cells with iDentification and qUantification sEparaTion (DUET).

ABSTRACT: While technologies for measuring transcriptomes in single cells have matured, methods for measuring proteins and their post-translational modification (PTM) states in single cells are still being actively developed. Unlike nucleic acids, proteins cannot be amplified, making detection of minute quantities from single cells difficult. Here, we develop a strategy to detect targeted protein and its PTM isoforms in single cells. We barcode the proteins from single cells by tagging them with oligonucleotides, pool barcoded cells together, run bulk gel electrophoresis to separate protein and its PTM isoform and quantify their abundances by sequencing the oligonucleotides associated with each protein species. We used this strategy, iDentification and qUantification sEparaTion (DUET), to measure hi

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7400673 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature