Parallelized disruption of prokaryotic and eukaryotic cells via miniaturized and automated bead mill.

ABSTRACT: The application of integrated microbioreactor systems is rapidly becoming of more interest to accelerate strain characterization and bioprocess development. However, available high-throughput screening capabilities are often limited to target extracellular compounds only. Consequently, there is a great demand for automated technologies allowing for miniaturized and parallel cell disruption providing access to intracellular measurements. In this study, a fully automated bead mill workflow was developed and validated for four different industrial platform organisms: Escherichia coli, Corynebacterium glutamicum, Saccharomyces cerevisiae, and Aspergillus niger. The workflow enables up to 48 parallel cell disruptions in microtiter plates and is applicable at-line to

SUBMITTER: Jansen RP 

PROVIDER: S-EPMC7401235 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature