Polymodal Sensory Transduction in Mouse Corneal Epithelial Cells.

ABSTRACT: Purpose:Contact lenses, osmotic stressors, and chemical burns may trigger severe discomfort and vision loss by damaging the cornea, but the signaling mechanisms used by corneal epithelial cells (CECs) to sense extrinsic stressors are not well understood. We therefore investigated the mechanisms of swelling, temperature, strain, and chemical transduction in mouse CECs. Methods:Intracellular calcium imaging in conjunction with electrophysiology, pharmacology, transcript analysis, immunohistochemistry, and bioluminescence assays of adenosine triphosphate (ATP) release were used to track mechanotransduction in dissociated CECs and epithelial sheets isolated from the mouse cornea. Results:The transient receptor potential vanilloid (TRPV) transcriptome in the mouse corneal epithelium is dominate

SUBMITTER: Lapajne L 

PROVIDER: S-EPMC7401707 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature