Mass Spectrometry Reveals α-2-HS-Glycoprotein as a Key Early Extracellular Matrix Protein for Conjunctival Cells.

ABSTRACT:

Purpose

To determine the composition of extracellular matrix (ECM) proteins secreted by a conjunctival epithelial cell line and to identify components that aid conjunctival epithelial cell culture.

Methods

Human conjunctival epithelial cell line (HCjE-Gi) cells were cultured in serum-free media and their ECM isolated using ammonium hydroxide. Growth characteristics were evaluated for fresh HCjE-Gi cells plated onto ECMs obtained from 3- to 28-day cell cultures. Mass spectrometry was used to characterize the ECM composition over 42 culture days. Cell adhesion and growth on pre-adsorbed fibronectin and α-2-HS-glycoprotein (α-2-HS-GP) were investigated.

Results

Day 3 ECM provided the best substrate for cell growth compared to ECM obtained from 5- to 28-day cell cultures.

SUBMITTER: Makuloluwa AK 

PROVIDER: S-EPMC7401837 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature