Abnormal Cone and Rod Photoreceptor Morphogenesis in gdf6a Mutant Zebrafish.

ABSTRACT:

Purpose

Analysis of photoreceptor morphology and gene expression in mispatterned eyes of zebrafish growth differentiation factor 6a (gdf6a) mutants.

Methods

Rod and cone photoreceptors were compared between gdf6a mutant and control zebrafish from larval to late adult stages using transgenic labels, immunofluorescence, and confocal microscopy, as well as by transmission electron microscopy. To compare transcriptomes between larval gdf6a mutant and control zebrafish, RNA-Seq was performed on isolated eyes.

Results

Although rod and cone photoreceptors differentiate in gdf6a mutant zebrafish, the cells display aberrant growth and morphology. The cone outer segments, the light-detecting sensory endings, are reduced in size in the mutant larvae and fail to recover to contro

SUBMITTER: Nadolski NJ 

PROVIDER: S-EPMC7401959 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature