Stromal Nerve Imaging and Tracking Using Micro-Optical Coherence Tomography.

ABSTRACT:

Purpose

To image, track and map the nerve fiber distribution in excised rabbit corneas over the entire stromal thickness using micro-optical coherence tomography (µOCT) to develop a screening tool for early peripheral neuropathy.

Methods

Excised rabbit corneas were consecutively imaged by a custom-designed µOCT prototype and a commercial laser scanning fluorescence confocal microscope. The µOCT images with a field of view of approximately 1 × 1 mm were recorded with axial and transverse resolutions of approximately 1 µm and approximately 4 µm, respectively. In the volumetric µOCT image data, network maps of hyper-reflective, branched structures traversing different stromal compartments were segmented using semiautomatic image processing algorithms. Furthermore, the same corn

SUBMITTER: Elhardt C 

PROVIDER: S-EPMC7401960 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature