The Paramecium histone chaperone Spt16-1 is required for Pgm endonuclease function in programmed genome rearrangements.

ABSTRACT: In Paramecium tetraurelia, a large proportion of the germline genome is reproducibly removed from the somatic genome after sexual events via a process involving small (s)RNA-directed heterochromatin formation and DNA excision and repair. How germline limited DNA sequences are specifically recognized in the context of chromatin remains elusive. Here, we use a reverse genetics approach to identify factors involved in programmed genome rearrangements. We have identified a P. tetraurelia homolog of the highly conserved histone chaperone Spt16 subunit of the FACT complex, Spt16-1, and show its expression is developmentally regulated. A functional GFP-Spt16-1 fusion protein localized exclusively in the nuclei where genome rearrangements take place. Gene silencing of Spt16-1 showed it is required

SUBMITTER: de Vanssay A 

PROVIDER: S-EPMC7402521 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature