Chemical proteomics tracks virus entry and uncovers NCAM1 as Zika virus receptor.

ABSTRACT: The outbreak of Zika virus (ZIKV) in 2016 created worldwide health emergency which demand urgent research efforts on understanding the virus biology and developing therapeutic strategies. Here, we present a time-resolved chemical proteomic strategy to track the early-stage entry of ZIKV into host cells. ZIKV was labeled on its surface with a chemical probe, which carries a photocrosslinker to covalently link virus-interacting proteins in living cells on UV exposure at different time points, and a biotin tag for subsequent enrichment and mass spectrometric identification of the receptor or other host proteins critical for virus internalization. We identified Neural Cell Adhesion Molecule (NCAM1) as a potential ZIKV receptor and further validated it through overexpression, knockout, and inhi

SUBMITTER: Srivastava M 

PROVIDER: S-EPMC7403387 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature