High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition.

ABSTRACT: Sperm cell motility and morphology observed under the bright field microscopy are the only criteria for selecting a particular sperm cell during Intracytoplasmic Sperm Injection (ICSI) procedure of Assisted Reproductive Technology (ART). Several factors such as oxidative stress, cryopreservation, heat, smoking and alcohol consumption, are negatively associated with the quality of sperm cell and fertilization potential due to the changing of subcellular structures and functions which are overlooked. However, bright field imaging contrast is insufficient to distinguish tiniest morphological cell features that might influence the fertilizing ability of sperm cell. We developed a partially spatially coherent digital holographic microscope (PSC-DHM) for quantitative phase imaging (QPI) in order

SUBMITTER: Butola A 

PROVIDER: S-EPMC7403412 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature