Omental adipocytes promote peritoneal metastasis of gastric cancer through the CXCL2-VEGFA axis.

ABSTRACT:

Background

Gastric cancer (GC) patients frequently develop peritoneal metastasis; however, the underlying mechanism remains unknown. We hypothesised that omental adipocytes (OmAd) trigger GC cells towards malignant activity to induce peritoneal metastasis.

Methods

We analysed interactions among human GC cells, endothelial cells and OmAd using a 3D co-culture system. We also employed a multipronged animal study, including subcutaneous and orthotopic tumours, and humanised omental adipose tissue models. Urinary levels of CXCL2 were analysed in human GC patients with and without peritoneal metastasis.

Results

Conditioned media derived from OmAd (OmAd-CM) promoted the proliferation, migration and capacity to induce angiogenesis of GC cells through AKT phosphorylation and

SUBMITTER: Natsume M 

PROVIDER: S-EPMC7403422 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature