Quantitative analysis of heparan sulfate using isotopically labeled calibrants.

ABSTRACT: Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight ¹³C-labeled disaccharide calibrants and one ¹³C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7403576 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature