Macrophage-driven nutrient delivery to phagosomal Staphylococcus aureus supports bacterial growth.

ABSTRACT: Staphylococcus aureus is a notorious pathogen causing significant morbidity and mortality worldwide. The ability of S. aureus to survive and replicate within phagocytes such as macrophages represents an important facet of immune evasion and contributes to pathogenesis. The mechanisms by which S. aureus acquires nutrients within host cells to support growth remain poorly characterized. Here, we demonstrate that macrophages infected with S. aureus maintain their dynamic ruffling behavior and consume macromolecules from the extracellular milieu. To support the notion that fluid-phase uptake by macrophages can provide S. aureus with nutrients, we utilized the pharmacological inhibitors PIK-III and Dynasore to impair uptake of extracellular macromolecules. Inhibitor treatment also impaired S. a

SUBMITTER: Flannagan RS 

PROVIDER: S-EPMC7403714 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature