Chronic myelomonocytic leukaemia stem cell transcriptomes anticipate disease morphology and outcome.

ABSTRACT:

Background

Chronic myelomonocytic leukaemia (CMML) is a clinically heterogeneous stem cell malignancy with overlapping features of myelodysplasia and myeloproliferation. Over 90% of patients carry mutations in epigenetic and/or splicing genes, typically detectable in the Lin^-CD34⁺CD38^- immunophenotypic stem cell compartment in which the leukaemia-initiating cells reside. Transcriptional dysregulation at the stem cell level is likely fundamental to disease onset and progression.

Methods

We performed single-cell RNA sequencing on 6826 Lin^-CD34⁺CD38^-stem cells from CMML patients and healthy controls using the droplet-based, ultra-high-throughput 10x platform.

Findings

We found substantial inter- and intra-

SUBMITTER: Wiseman DH 

PROVIDER: S-EPMC7403890 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature