Activation of NOTCH1 by Shear Force Elicits Immediate Cytokine Expression in Human Chondrocytes.

ABSTRACT: Osteoarthritis is caused by overloading of joints and is characterized by inflammation-induced disruption of cartilage structure. Current treatment strategy aims to relieve inflammation and prevent further deterioration of joint function. However, how mechanical force leads to inflammation and deterioration of chondrocyte function still remains incompletely understood. To explore the force-regulated molecular mechanism, an in vitro hydraulic shear force experiment to simulate the condition of force loading was required. The result demonstrated that multiple cytokines and immune regulators, including interleukin 8, interferon β, TRAF1 and TNFAIP3, were significantly increased by shear force within two hours of treatment. Moreover, JAG1 and HES1 were drastically upregulated as well, suggesti

SUBMITTER: Cheng HJ 

PROVIDER: S-EPMC7404062 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature