Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks.

ABSTRACT:

Background

Toxoplasma gondii is a major cause of abortion in small ruminants and presents a zoonotic risk when undercooked meat containing cysts is consumed. The aim of the present study was to investigate the genetic diversity among the T. gondii strains circulating in ovine livestock in Spain.

Methods

Selected samples collected from abortion outbreaks due to toxoplasmosis (n = 31) and from chronically infected adult sheep at slaughterhouses (n = 50) in different Spanish regions were bioassayed in mice, aiming at parasite isolation. In addition, all original clinical samples and the resulting isolates were genotyped by multi-nested PCR-RFLP analysis of 11 molecular markers and by PCR-DNA sequencing of portions of the SAG3, GRA6 and GRA7 genes.

Results

As a result, 30

SUBMITTER: Fernandez-Escobar M 

PROVIDER: S-EPMC7404076 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature