Two Clubroot-Resistance Genes, Rcr3 and Rcr9^wa, Mapped in Brassica rapa Using Bulk Segregant RNA Sequencing.

ABSTRACT: Genetic resistance is widely used to manage clubroot (Plasmodiophora brassicae) in brassica crops, but new pathotypes have recently been identified on canola (Brassica napus) on the Canadian prairies. Resistance effective against both the most prevalent pathotype (3H, based on the Canadian Clubroot Differential system) and the new pathotypes is needed. BC₁ plants of Brassica rapa from a cross of line 96-6990-2 (clubroot resistance originating from turnip cultivar 'Waaslander') and a susceptible doubled-haploid line, ACDC, exhibited a 1:1 segregation for resistance against pathotypes 3H and 5X. A resistance gene designated as Rcr3 was mapped initially based on the percentage of polymorphic variants using bulked segregant RNA sequencing (BSR-Seq) and fu

SUBMITTER: Karim MM 

PROVIDER: S-EPMC7404267 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature