No evidence of SARS-CoV-2 RNA among blood donors: A multicenter study in Hubei, China.

ABSTRACT: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA could be detected in the blood of infected cases. From February 9, all blood establishments in Hubei province, China, implemented nucleic acid testing (NAT) for SARS-CoV-2 RNA among blood donors to ensure blood safety.

Study design and methods

Nucleic acid test screening individually (ID) or by minipool (MP) testing was performed according to the manufacturer's instructions. Inactivated culture supernatant of SARS-CoV-2-infected Vero cells was quantified by droplet digital polymerase chain reaction (ddPCR) and series diluted with negative plasma to evaluate the assay's performance.

Results

The limit of detection of the kit for MP testing was 62.94 and 33.14 copies/mL for N and ORF1ab region, respectively. ID tes

SUBMITTER: Chang L 

PROVIDER: S-EPMC7404423 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature